褐黄血蜱卵蛋白质组分析

doi:10.11843/j.issn.0366-6964.2019.03.018

Abstract

Abstract:

The protein components of Haemaphysalis flava eggs were investigated, and the key nutrients were analyzed in the development of embryo, in order to lay a foundation for screening antigen molecules that can interfere with embryonic development. Firstly, the egg protein components of fresh Haemaphysalis flava were analyzed by LC-MS/MS, and based on the salivary gland transcriptome translation library, the midgut transcriptome translation library and the Uniprot database, each protein components were identified. A total of 221 unique peptides were detected from the yolk protein extract of Haemaphysalis flava, and 53 kinds of proteins were identified, among which 12 were highly confident proteins. The functional proteins that can be identified were actin and vitellogenin 2 (Vg-2). Vg-2 is completely same with the primary structure of vitellins (Vn). The protoplasts of the eggs contain less protein, and there is only one type of vitellogenin (Vg-2).

CLC Number:

S852.746

XU Lü,CHENG Tianyin. Proteomics Analysis of Haemaphysalis flava Eggs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 627-636.

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Proteomics Analysis of Haemaphysalis flava Eggs

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